BAIT
RHOBTB1
Rho-related BTB domain containing 1
GO Process (2)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
MADD
DENN, IG20, RAB3GEP
MAP-kinase activating death domain
GO Process (9)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- activation of MAPK activity [IMP]
- cell surface receptor signaling pathway [TAS]
- execution phase of apoptosis [IMP]
- positive regulation of Rab GTPase activity [IDA, IMP]
- regulation of Rab protein signal transduction [IDA]
- regulation of apoptotic process [IMP]
- regulation of cell cycle [IMP]
- regulation of extrinsic apoptotic signaling pathway [IDA]
- regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RHOBTB1 wild type bait, interaction in Hela cells
Curated By
- BioGRID