RAT1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SSA1
Gene Ontology Biological Process
- SRP-dependent cotranslational protein targeting to membrane, translocation [IDA]
- clathrin coat disassembly [IDA]
- cytoplasmic translation [IMP]
- protein folding [IDA]
- protein import into nucleus, translocation [IDA]
- protein refolding [IDA]
- protein targeting to mitochondrion [IMP]
- stress granule disassembly [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
An atlas of chaperone-protein interactions in Saccharomyces cerevisiae: implications to protein folding pathways in the cell.
Molecular chaperones are known to be involved in many cellular functions, however, a detailed and comprehensive overview of the interactions between chaperones and their cofactors and substrates is still absent. Systematic analysis of physical TAP-tag based protein-protein interactions of all known 63 chaperones in Saccharomyces cerevisiae has been carried out. These chaperones include seven small heat-shock proteins, three members of ... [more]
Throughput
- High Throughput
Additional Notes
- The authors note that 21,687 interactions were identified as high confidence based on experimental scores and the distribution of scores in reference databases. Only 1420 high confidence interactions were submitted to BioGRID by the authors because they met the additional criteria of containing reciprocal interactors in terms of the bait-prey relationship.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SSA1 RAT1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 333627 | |
| RAT1 SSA1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1523 | BioGRID | 2015310 |
Curated By
- BioGRID