BAIT
RAC1
Rac-1, TC-25, p21-Rac1, MIG5
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
GO Process (41)
GO Function (6)
GO Component (10)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- GTP catabolic process [TAS]
- T cell costimulation [TAS]
- actin cytoskeleton organization [IGI]
- actin filament polymerization [TAS]
- anatomical structure morphogenesis [TAS]
- apoptotic signaling pathway [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell adhesion [TAS]
- cell motility [IDA]
- cell-matrix adhesion [NAS]
- cellular component movement [TAS]
- inflammatory response [TAS]
- innate immune response [TAS]
- intracellular signal transduction [TAS]
- lamellipodium assembly [IMP]
- localization within membrane [IMP]
- negative regulation of interleukin-23 production [IDA]
- negative regulation of receptor-mediated endocytosis [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet activation [TAS]
- positive regulation of Rho protein signal transduction [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of cell-substrate adhesion [IGI]
- positive regulation of focal adhesion assembly [IDA]
- positive regulation of lamellipodium assembly [IDA, IMP]
- positive regulation of neutrophil chemotaxis [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of stress fiber assembly [IDA]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of cell migration [IMP]
- regulation of defense response to virus by virus [TAS]
- regulation of hydrogen peroxide metabolic process [TAS]
- regulation of respiratory burst [IDA]
- response to wounding [TAS]
- ruffle organization [IDA, TAS]
- semaphorin-plexin signaling pathway [ISS]
- substrate adhesion-dependent cell spreading [IMP]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
ITGB1BP1
ICAP-1A, ICAP-1B, ICAP-1alpha, ICAP1, ICAP1A, ICAP1B
integrin beta 1 binding protein 1
GO Process (34)
GO Function (5)
GO Component (12)
Gene Ontology Biological Process
- activation of protein kinase B activity [IDA]
- blood vessel endothelial cell proliferation involved in sprouting angiogenesis [IDA]
- cell migration [TAS]
- cell-matrix adhesion [IDA]
- cellular response to fibroblast growth factor stimulus [IDA]
- cellular response to vascular endothelial growth factor stimulus [IDA]
- integrin activation [ISS]
- integrin-mediated signaling pathway [IDA]
- intracellular signal transduction [TAS]
- myoblast migration [ISS]
- negative regulation of ERK1 and ERK2 cascade [IDA]
- negative regulation of cell adhesion involved in substrate-bound cell migration [ISS]
- negative regulation of cell migration involved in sprouting angiogenesis [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of fibroblast migration [ISS]
- negative regulation of focal adhesion assembly [IDA]
- negative regulation of protein binding [IDA]
- negative regulation of protein kinase activity [IDA]
- negative regulation of protein targeting to membrane [IDA]
- negative regulation of substrate adhesion-dependent cell spreading [IDA]
- positive regulation of Notch signaling pathway [IDA]
- positive regulation of cell proliferation [IDA]
- positive regulation of endothelial cell migration [IDA]
- positive regulation of protein kinase B signaling [IDA]
- positive regulation of protein targeting to membrane [ISS]
- positive regulation of stress fiber assembly [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- protein transport [IDA]
- receptor clustering [ISS]
- regulation of Cdc42 GTPase activity [IDA]
- regulation of blood vessel size [IDA]
- regulation of cell adhesion mediated by integrin [ISS]
- regulation of integrin-mediated signaling pathway [ISS]
- tube formation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms.
Guanine nucleotide exchange factors (RhoGEFs) and GTPase-activating proteins (RhoGAPs) coordinate the activation state of the Rho family of GTPases for binding to effectors. Here, we exploited proximity-dependent biotinylation to systematically define the Rho family proximity interaction network from 28 baits to produce 9,939 high-confidence proximity interactions in two cell lines. Exploiting the nucleotide states of Rho GTPases, we revealed the ... [more]
Nat. Cell Biol. Dec. 23, 2019; (); [Pubmed: 31871319]
Throughput
- High Throughput
Additional Notes
- AvgP greater or equal to 0.95 assessed by SAINT express
- BioID
- RAC1-G15A nucleotide free mutant bait, interaction in Hela cells
Curated By
- BioGRID