BAIT

KCTD17

RP5-1170K4.4
potassium channel tetramerization domain containing 17
GO Process (0)
GO Function (2)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

GNB4

CMTD1F, RP11-145M9.4
guanine nucleotide binding protein (G protein), beta polypeptide 4
GO Process (4)
GO Function (1)
GO Component (3)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human Multisubunit E3 Ubiquitin Ligase Required for Heterotrimeric G-Protein ?-Subunit Ubiquitination and Downstream Signaling.

Young BD, Sha J, Vashisht AA, Wohlschlegel JA

G-protein-coupled receptors (GPCRs) initiate intracellular signaling events through heterotrimeric G-protein ?-subunits (G?) and the ??-subunit dimer (G??). In this study, we utilized mass spectrometry to identify novel regulators of G?? signaling in human cells. This prompted our characterization of KCTD2 and KCTD5, two related potassium channel tetramerization domain (KCTD) proteins that specifically recognize G??. We demonstrated that these KCTD proteins ... [more]

J Proteome Res Dec. 03, 2020; 20(9);4318-4330 [Pubmed: 34342229]

Throughput

  • High Throughput

Curated By

  • BioGRID