BAIT

SUMO2

HSMT3, SMT3B, SMT3H2, SUMO3, Smt3A

small ubiquitin-like modifier 2

GO Process (4)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]

post-translational protein modification [TAS]

protein sumoylation [IDA, TAS]

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]
protein binding [IPI]
ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

nucleoplasm [TAS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

SALL1

HEL-S-89, HSAL1, Sal-1, TBS, ZNF794

spalt-like transcription factor 1

GO Process (25)

GO Function (5)

GO Component (5)

Gene Ontology Biological Process

adrenal gland development [IEP]

branching involved in ureteric bud morphogenesis [ISS]

embryonic digestive tract development [IMP]

embryonic digit morphogenesis [IMP]

gonad development [IEP]

heart development [IMP]

histone deacetylation [ISS]

inductive cell-cell signaling [ISS]

kidney development [IMP]

kidney epithelium development [ISS]

limb development [IMP]

mesenchymal to epithelial transition involved in metanephros morphogenesis [IEP]

negative regulation of transcription from RNA polymerase II promoter [IDA]

negative regulation of transcription, DNA-templated [IDA]

olfactory bulb interneuron differentiation [ISS]

olfactory bulb mitral cell layer development [IMP]

olfactory nerve development [ISS]

outer ear morphogenesis [IMP]

pituitary gland development [IEP]

positive regulation of Wnt signaling pathway [IDA]

positive regulation of transcription from RNA polymerase II promoter [IDA]

positive regulation of transcription, DNA-templated [IDA]

ureteric bud development [ISS]

ureteric bud invasion [ISS]

ventricular septum development [ISS]

Gene Ontology Molecular Function

DNA binding [NAS]
beta-catenin binding [IDA]
histone deacetylase activity [ISS]
protein binding [IPI]
sequence-specific DNA binding transcription factor activity [NAS]

Gene Ontology Cellular Component

NuRD complex [ISS]

chromocenter [IDA]

cytoplasm [IDA]

heterochromatin [IDA]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD

The fast dynamics and reversibility of posttranslational modifications by the ubiquitin family pose significant challenges for research. Here we present SUMO-ID, a technology that merges proximity biotinylation by TurboID and protein-fragment complementation to find SUMO-dependent interactors of proteins of interest. We develop an optimized split-TurboID version and show SUMO interaction-dependent labelling of proteins proximal to PML and RANGAP1. SUMO-dependent interactors ... [more]

Nat Commun Dec. 18, 2020; 12(1);6671 [Pubmed: 34795231]

Throughput

Low Throughput

Additional Notes

#LPPI Likely protein-protein interaction and
#LPTM Likely post-translational modification
TurboID

Curated By

BioGRID