Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

Li S, Zhang L, Zhang G, Shangguan G, Hou X, Duan W, Xi Y, Xu N, Zhang B, Dong J, Wang Y, Cui W, Chen S

Autophagy is a conserved biological process that maintains cell homeostasis by targeting macromolecules for lysosome-mediated degradation. The levels of autophagy are relatively lower under normal conditions than under stress conditions (e.g., starvation), as autophagy is usually stimulated after multiple stresses. However, many autophagy-related regulators are still expressed under normal conditions. Although these regulators have been studied deeply in autophagy regulation, ... [more]

iScience Nov. 19, 2021; 24(11);103296 [Pubmed: 34755101]

Throughput

  • Low Throughput

Curated By

  • BioGRID