BAIT

LMNA

cb948, fk66d12, wu:fk66d12
lamin A
GO Process (2)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Danio rerio
PREY

PTGIS

PGIS, ptgisl
prostaglandin I2 (prostacyclin) synthase
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Danio rerio

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos.

Rosenthal SM, Misra T, Abdouni H, Branon TC, Ting AY, Scott IC, Gingras AC

Understanding how proteins are organized in compartments is essential to elucidating their function. While proximity-dependent approaches such as BioID have enabled a massive increase in information about organelles, protein complexes, and other structures in cell culture, to date there have been only a few studies on living vertebrates. Here, we adapted proximity labeling for protein discovery in vivo in the vertebrate ... [more]

Mol Cell Proteomics Aug. 01, 2021; 20();100128 [Pubmed: 34332124]

Throughput

  • High Throughput

Additional Notes

  • Applied SAINT express scoring
  • BioID experiments in transgenic system (controlled by heat shock promoter)
  • High-confidence proximal partners had a BFDR cutoff of equal or less than 1 percent
  • Interactions identified using TurboID-LMNA
  • Interactions identified using TurboID-LMNA at 48h
  • mRNA injection-based BioID experiments

Curated By

  • BioGRID