BAIT
LMNA
cb948, fk66d12, wu:fk66d12
lamin A
GO Process (2)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Danio rerio
PREY
DOT1L
si:dkey-211f22.1, DKEY-211F22.1
DOT1-like histone H3K79 methyltransferase
GO Process (2)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Danio rerio
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A Toolbox for Efficient Proximity-Dependent Biotinylation in Zebrafish Embryos.
Understanding how proteins are organized in compartments is essential to elucidating their function. While proximity-dependent approaches such as BioID have enabled a massive increase in information about organelles, protein complexes, and other structures in cell culture, to date there have been only a few studies on living vertebrates. Here, we adapted proximity labeling for protein discovery in vivo in the vertebrate ... [more]
Mol Cell Proteomics Aug. 01, 2021; 20();100128 [Pubmed: 34332124]
Throughput
- High Throughput
Additional Notes
- Applied SAINT express scoring
- High-confidence proximal partners had a BFDR cutoff of equal or less than 1 percent
- Interactions identified using TurboID-LMNA at 48h
- mRNA injection-based BioID experiments
Curated By
- BioGRID