BAIT

HNRNPH1

HNRPH, HNRPH1, hnRNPH
heterogeneous nuclear ribonucleoprotein H1 (H)
GO Process (5)
GO Function (4)
GO Component (4)
Homo sapiens
PREY

HNRNPF

HNRPF, OK/SW-cl.23, mcs94-1
heterogeneous nuclear ribonucleoprotein F
GO Process (5)
GO Function (4)
GO Component (4)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The small RNA mascRNA differentially regulates TLR-induced proinflammatory and antiviral responses.

Sun T, Wei C, Wang D, Wang X, Wang J, Hu Y, Mao X

MALAT1-associated small cytoplasmic RNA (mascRNA) is a highly conserved transfer RNA-like (tRNA-like) noncoding RNA whose function remains largely unknown. We show here that this small RNA molecule played a role in the stringent control of TLR-mediated innate immune responses. mascRNA inhibited activation of NF-?B and mitogen-activated protein kinase (MAPK) signaling and the production of inflammatory cytokines in macrophages stimulated with ... [more]

JCI Insight Dec. 08, 2020; 6(21); [Pubmed: 34582376]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HNRNPH1 HNRNPF
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3358359
HNRNPH1 HNRNPF
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.778BioGRID
741365
HNRNPH1 HNRNPF
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3442076
HNRNPH1 HNRNPF
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
HNRNPH1 HNRNPF
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2738613

Curated By

  • BioGRID