BAIT

HA2

AHA2, F9N11.40, F9N11_40, H(+)-ATPase 2, P-TYPE H(+)-ATPASE ISOFORM 2, PLASMA MEMBRANE PROTON ATPASE 2, PMA2, AT4G30190
H(+)-ATPase 2
Arabidopsis thaliana (Columbia)
PREY

GF14 PHI

14-3-3 PROTEIN G-BOX FACTOR14 PHI, 14-3-3PHI, GENERAL REGULATORY FACTOR 4, GF14 protein phi chain, GRF4, T32G9.30, T32G9_30, AT1G35160
14-3-3-like protein GF14 phi
GO Process (1)
GO Function (2)
GO Component (6)
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The 14-3-3 protein interacts directly with the C-terminal region of the plant plasma membrane H(+)-ATPase.

Jahn T, Fuglsang AT, Olsson A, Bruentrup IM, Collinge DB, Volkmann D, Sommarin M, Palmgren MG, Larsson C

Accumulating evidence suggests that 14-3-3 proteins are involved in the regulation of plant plasma membrane H(+)-ATPase activity. However, it is not known whether the 14-3-3 protein interacts directly or indirectly with the H(+)-ATPase. In this study, detergent-solubilized plasma membrane H(+)-ATPase isolated from fusicoccin-treated maize shoots was copurified with the 14-3-3 protein (as determined by protein gel blotting), and the H(+)-ATPase ... [more]

Plant Cell Oct. 01, 1997; 9(10);1805-14 [Pubmed: 9368417]

Throughput

  • Low Throughput

Curated By

  • BioGRID