BAIT
MAF1
RNA polymerase III-inhibiting protein MAF1, L000002614, YDR005C
Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
GO Process (2)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
NSR1
SHE5, L000001278, YGR159C
Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis
GO Process (2)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Growth Defect
A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.
Publication
Genetic interactions of MAF1 identify a role for Med20 in transcriptional repression of ribosomal protein genes.
Transcriptional repression of ribosomal components and tRNAs is coordinately regulated in response to a wide variety of environmental stresses. Part of this response involves the convergence of different nutritional and stress signaling pathways on Maf1, a protein that is essential for repressing transcription by RNA polymerase (pol) III in Saccharomyces cerevisiae. Here we identify the functions buffering yeast cells that ... [more]
PLoS Genet. Jul. 01, 2008; 4(7);e1000112 [Pubmed: 18604275]
Throughput
- Low Throughput
Ontology Terms
- phenotype: vegetative growth (APO:0000106)
- phenotype: fitness (APO:0000216)
Curated By
- BioGRID