KRAS
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein phosphorylation [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
BIRC5
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [IDA]
- cell division [IMP]
- cytokinesis [IMP]
- establishment of chromosome localization [IMP]
- inhibition of cysteine-type endopeptidase activity involved in apoptotic process [IBA]
- mitotic cell cycle [TAS]
- mitotic nuclear division [TAS]
- negative regulation of apoptotic process [IDA, IMP]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, IMP]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of cell proliferation [TAS]
- positive regulation of exit from mitosis [IMP]
- positive regulation of mitotic cell cycle [IMP]
- protein complex localization [IMP]
- protein phosphorylation [IDA]
- protein ubiquitination [IBA]
- regulation of signal transduction [IBA]
- spindle assembly involved in mitosis [IBA]
- spindle checkpoint [IMP]
Gene Ontology Molecular Function- Ran GTPase binding [IPI]
- chaperone binding [IPI]
- cobalt ion binding [NAS]
- cofactor binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- enzyme binding [IPI]
- identical protein binding [IPI]
- microtubule binding [IDA, TAS]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tubulin binding [IDA]
- ubiquitin-protein transferase activity [IBA]
- zinc ion binding [IDA, NAS]
- Ran GTPase binding [IPI]
- chaperone binding [IPI]
- cobalt ion binding [NAS]
- cofactor binding [IDA]
- cysteine-type endopeptidase inhibitor activity involved in apoptotic process [IMP]
- enzyme binding [IPI]
- identical protein binding [IPI]
- microtubule binding [IDA, TAS]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- protein homodimerization activity [IDA, IPI]
- tubulin binding [IDA]
- ubiquitin-protein transferase activity [IBA]
- zinc ion binding [IDA, NAS]
Gene Ontology Cellular Component
- centriole [IDA]
- chromosome passenger complex [IPI]
- chromosome, centromeric region [IDA]
- condensed chromosome kinetochore [IDA]
- cytoplasm [IDA]
- cytoplasmic microtubule [IDA]
- cytosol [IDA, TAS]
- interphase microtubule organizing center [IDA]
- midbody [IDA]
- nuclear chromosome [IDA]
- nucleus [IDA]
- spindle microtubule [IDA]
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Here, genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies ... [more]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: PC9-Cas9-KRASG12V
- Experimental Setup: Negative selection in the presence of 40 nM erlotinib
- GIST: A-phenotypic negative genetic interaction
- Library: Brunello Library
- Significance Threshold:
- CS
- >0.5 and p<0.05
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KRAS BIRC5 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | High | - | BioGRID | 2619371 |
Curated By
- BioGRID