PREY

CHMP6

VPS20
charged multivesicular body protein 6
GO Process (5)
GO Function (2)
GO Component (3)
Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH

CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Here, genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies ... [more]

Nat Commun Dec. 09, 2020; 12(1);4789 [Pubmed: 34373451]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

  • CRISPR GI screen
  • Cell Line: PC9-Cas9-KRASG12V
  • Experimental Setup: Negative selection in the presence of 40 nM erlotinib
  • GIST: A-phenotypic negative genetic interaction
  • Library: Brunello Library
  • Significance Threshold:
  • CS
  • >0.5 and p<0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KRAS CHMP6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Low-BioGRID
2548751
KRAS CHMP6
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High5.9564BioGRID
2604615

Curated By

  • BioGRID