BAIT
KRAS
C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Kirsten rat sarcoma viral oncogene homolog
GO Process (16)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein phosphorylation [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DNA2
DNA2L, hDNA2, RP11-9E13.1
DNA replication helicase/nuclease 2
GO Process (17)
GO Function (10)
GO Component (4)
Gene Ontology Biological Process
- ATP catabolic process [IDA, TAS]
- DNA catabolic process, endonucleolytic [IDA]
- DNA double-strand break processing [IDA]
- DNA duplex unwinding [IDA]
- DNA replication [IMP]
- DNA replication checkpoint [IMP]
- DNA replication, Okazaki fragment processing [IDA]
- DNA replication, removal of RNA primer [IDA]
- DNA strand elongation involved in DNA replication [TAS]
- base-excision repair [IDA]
- mitochondrial DNA repair [IDA]
- mitochondrial DNA replication [IDA]
- mitotic cell cycle [TAS]
- positive regulation of DNA replication [IDA]
- telomere maintenance [TAS]
- telomere maintenance via recombination [TAS]
- telomere maintenance via semi-conservative replication [TAS]
Gene Ontology Molecular Function- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
- 5'-3' DNA helicase activity [IDA]
- 5'-flap endonuclease activity [IDA]
- ATPase activity [TAS]
- DNA binding [IDA]
- DNA helicase activity [IDA]
- helicase activity [TAS]
- nuclease activity [IDA]
- protein binding [IPI]
- single-stranded DNA-dependent ATPase activity [IDA]
- site-specific endodeoxyribonuclease activity, specific for altered base [IDA]
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Here, genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies ... [more]
Nat Commun Dec. 09, 2020; 12(1);4789 [Pubmed: 34373451]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: PC9-Cas9-KRASG12V
- Experimental Setup: Negative selection in the presence of 40 nM erlotinib
- GIST: A-phenotypic negative genetic interaction
- Library: Brunello Library
- Significance Threshold:
- CS
- >0.5 and p<0.05
Curated By
- BioGRID