BAIT
KRAS
C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Kirsten rat sarcoma viral oncogene homolog
GO Process (16)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of cell proliferation [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of protein phosphorylation [IMP]
- small GTPase mediated signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PNPT1
COXPD13, DFNB70, OLD35, PNPASE, old-35
polyribonucleotide nucleotidyltransferase 1
GO Process (24)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- RNA catabolic process [IDA]
- RNA import into mitochondrion [IDA]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- RNA polyadenylation [IDA]
- cellular response to interferon-beta [IDA]
- cellular response to oxidative stress [IDA]
- mRNA catabolic process [IDA]
- mitochondrial RNA 3'-end processing [IMP]
- mitochondrial RNA 5'-end processing [IMP]
- mitochondrial RNA catabolic process [IDA]
- mitochondrial mRNA catabolic process [IDA]
- mitochondrial mRNA polyadenylation [IMP]
- mitochondrion morphogenesis [ISS]
- mitotic cell cycle arrest [IDA]
- negative regulation of growth [IDA]
- nuclear polyadenylation-dependent mRNA catabolic process [IDA]
- positive regulation of mRNA catabolic process [IMP]
- positive regulation of miRNA catabolic process [IDA]
- positive regulation of mitochondrial RNA catabolic process [IDA]
- protein homooligomerization [IDA]
- protein homotrimerization [IDA]
- rRNA import into mitochondrion [IDA]
- regulation of cellular respiration [ISS]
- regulation of cellular senescence [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.
CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Here, genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies ... [more]
Nat Commun Dec. 09, 2020; 12(1);4789 [Pubmed: 34373451]
Throughput
- High Throughput
Ontology Terms
- growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: PC9-Cas9-KRASG12V
- Experimental Setup: Negative selection in the presence of 40 nM erlotinib
- GIST: A-phenotypic negative genetic interaction
- Library: Brunello Library
- Significance Threshold:
- CS
- >0.5 and p<0.05
Curated By
- BioGRID