BAIT

EP300

KAT3B, RSTS2, p300, RP1-85F18.1
E1A binding protein p300
GO Process (29)
GO Function (15)
GO Component (3)

Gene Ontology Cellular Component

Homo sapiens
PREY

NAP1L1

NAP1, NAP1L, NRP
nucleosome assembly protein 1-like 1
GO Process (2)
GO Function (2)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivators.

Shikama N, Chan HM, Krstic-Demonacos M, Smith L, Lee CW, Cairns W, La Thangue NB

The p300/CREB-binding protein (CBP) family of proteins consists of coactivators that influence the activity of a wide variety of transcription factors. Although the mechanisms that allow p300/CBP proteins to achieve transcriptional control are not clear, it is believed that the regulation of chromatin is an important aspect of the process. Here, we describe a new level of p300-dependent control mediated ... [more]

Mol. Cell. Biol. Dec. 01, 2000; 20(23);8933-43 [Pubmed: 11073993]

Throughput

  • Low Throughput

Additional Notes

  • mammalian two-hybrid

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EP300 NAP1L1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
2751635
EP300 NAP1L1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
EP300 NAP1L1
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
2751632

Curated By

  • BioGRID