Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Vichas A, Riley AK, Nkinsi NT, Kamlapurkar S, Parrish PCR, Lo A, Duke F, Chen J, Fung I, Watson J, Rees M, Gabel AM, Thomas JD, Bradley RK, Lee JK, Hatch EM, Baine MK, Rekhtman N, Ladanyi M, Piccioni F, Berger AH

CRISPR-based cancer dependency maps are accelerating advances in cancer precision medicine, but adequate functional maps are limited to the most common oncogenes. To identify opportunities for therapeutic intervention in other rarer subsets of cancer, we investigate the oncogene-specific dependencies conferred by the lung cancer oncogene, RIT1. Here, genome-wide CRISPR screening in KRAS, EGFR, and RIT1-mutant isogenic lung cancer cells identifies ... [more]

Nat Commun Dec. 09, 2020; 12(1);4789 [Pubmed: 34373451]

Throughput

High Throughput

Ontology Terms

phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]

Additional Notes

CRISPR GI screen
Cell Line: PC9-Cas9-RIT1M90I
Experimental Setup: Negative selection in the presence of 40 nM erlotinib
GIST: A-phenotypic negative genetic interaction
Library: Brunello Library
Significance Threshold:
CS
>0.5 and p<0.05

Curated By

BioGRID

Interaction Summary

RIT1

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin binding [TAS]protein binding [IPI]

DNM2

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [NAS]GTPase activity [NAS, TAS]SH3 domain binding [IDA]enzyme binding [NAS]microtubule binding [NAS]protein binding [IPI]

Gene Ontology Cellular Component

Negative Genetic