BAIT
CIPK1
CBL-interacting protein kinase 1, SNF1-RELATED PROTEIN KINASE 3.16, SnRK3.16, AT3G17510
CBL-interacting serine/threonine-protein kinase 1
GO Process (3)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Arabidopsis thaliana (Columbia)
PREY
CBL5
F3D13.2, F3D13_2, calcineurin B-like protein 5, AT4G01420
calcineurin B-like protein 5
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Arabidopsis thaliana (Columbia)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases.
The Arabidopsis calcineurin B-like calcium sensor proteins (AtCBLs) interact with a group of serine-threonine protein kinases (AtCIPKs) in a calcium-dependent manner. Here we identify a 24 amino acid domain (NAF domain) unique to these kinases as being required and sufficient for interaction with all known AtCBLs. Mutation of conserved residues either abolished or significantly diminished the affinity of AtCIPK1 for ... [more]
EMBO J. Mar. 01, 2001; 20(5);1051-63 [Pubmed: 11230129]
Throughput
- Low Throughput
Curated By
- BioGRID