An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma.

Mahlokozera T, Patel B, Chen H, Desouza P, Qu X, Mao DD, Hafez D, Yang W, Taiwo R, Paturu M, Salehi A, Gujar AD, Dunn GP, Mosammaparast N, Petti AA, Yano H, Kim AH

The pluripotency transcription factor SOX2 is essential for the maintenance of glioblastoma stem cells (GSC), which are thought to underlie tumor growth, treatment resistance, and recurrence. To understand how SOX2 is regulated in GSCs, we utilized a proteomic approach and identified the E3 ubiquitin ligase TRIM26 as a direct SOX2-interacting protein. Unexpectedly, we found TRIM26 depletion decreased SOX2 protein levels ... [more]

Nat Commun Dec. 03, 2020; 12(1);6321 [Pubmed: 34732716]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SOX2 ATAD3A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.		High	-	BioGRID	3321457

Curated By

BioGRID

Interaction Summary

SOX2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA, NAS]miRNA binding [IDA]protein binding [IPI]sequence-specific DNA binding [IDA]sequence-specific DNA binding transcription factor activity [IDA, NAS]transcription regulatory region DNA binding [IDA]

Gene Ontology Cellular Component

ATAD3A