BAIT

ABI2

ABA INSENSITIVE 2, AtABI2, MHM17.19, MHM17_19, PROTEIN PHOSPHATASE 2C, AT5G57050
protein phosphatase 2C 77
Arabidopsis thaliana (Columbia)
PREY

CIPK15

ATPK10, CBL-interacting protein kinase 15, PKS3, PROTEIN KINASE 10, SERINE/THREONINE PROTEIN KINASE 10, SIP2, SMALL AND BASIC INTRINSIC PROTEIN 2, SNF1-RELATED PROTEIN KINASE 3.1, SNRK3.1, SOS3-INTERACTING PROTEIN 2, T20L15.80, T20L15_80, AT5G01810
CBL-interacting serine/threonine-protein kinase 15
GO Process (2)
GO Function (2)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling in Arabidopsis.

Guo Y, Xiong L, Song CP, Gong D, Halfter U, Zhu JK

The phytohormone abscisic acid (ABA) triggers an oscillation in the cytosolic Ca(2+) concentration, which is then perceived by unknown Ca(2+) binding proteins to initiate a series of signaling cascades that control many physiological processes, including adaptation to environmental stress. We report here that a Ca(2+) binding protein, SCaBP5, and its interacting protein kinase, PKS3, function as global regulators of ABA ... [more]

Dev. Cell Aug. 01, 2002; 3(2);233-44 [Pubmed: 12194854]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIPK15 ABI2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
CIPK15 ABI2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID