Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

COI1 links jasmonate signalling and fertility to the SCF ubiquitin-ligase complex in Arabidopsis.

Devoto A, Nieto-Rostro M, Xie D, Ellis C, Harmston R, Patrick E, Davis J, Sherratt L, Coleman M, Turner JG

Jasmonates (JAs) regulate Arabidopsis thaliana wound and defence responses, pollen development, and stress-related growth inhibition. Significantly, each of these responses requires COI1, an F-box protein. Other F-box proteins interact with SKP1 and cullin proteins to form SCF complexes that selectively recruit regulatory proteins targeted for ubiquitination. To determine whether COI1 also functions in an SCF complex, we have characterized Arabidopsis ... [more]

Plant J. Nov. 01, 2002; 32(4);457-66 [Pubmed: 12445118]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TIR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

auxin binding [IDA, IGI]inositol hexakisphosphate binding [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IC, ISS]

Gene Ontology Cellular Component

CIP1