BAIT

CUL1

ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1, AT4G02570
cullin 1
Arabidopsis thaliana (Columbia)
PREY

FUS12

ATCSN2, CONSTITUTIVE PHOTOMORPHOGENIC 12, COP12, CSN2, FUSCA 12, T20P8.4, T20P8_4, AT2G26990
protein CONSTITUTIVE PHOTOMORPHOGENIC 12
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of the last subunit of the Arabidopsis COP9 signalosome: implications for the overall structure and origin of the complex.

Serino G, Su H, Peng Z, Tsuge T, Wei N, Gu H, Deng XW

The COP9 signalosome (CSN) is an evolutionarily conserved protein complex that resembles the lid subcomplex of proteasomes. Through its ability to regulate specific proteasome-mediated protein degradation events, CSN controls multiple aspects of development. Here, we report the cloning and characterization of AtCSN2, the last uncharacterized CSN subunit from Arabidopsis. We show that the AtCSN2 gene corresponds to the previously identified ... [more]

Plant Cell Mar. 01, 2003; 15(3);719-31 [Pubmed: 12615944]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CUL1 FUS12
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BAR
-
CUL1 FUS12
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID