BAIT
CALM3
HEL-S-72, PHKD, PHKD3
calmodulin 3 (phosphorylase kinase, delta)
GO Process (43)
GO Function (11)
GO Component (13)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- G-protein coupled receptor signaling pathway [TAS]
- activation of phospholipase C activity [TAS]
- blood coagulation [TAS]
- carbohydrate metabolic process [TAS]
- detection of calcium ion [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose metabolic process [TAS]
- glycogen catabolic process [TAS]
- innate immune response [TAS]
- inositol phosphate metabolic process [TAS]
- membrane organization [TAS]
- muscle contraction [TAS]
- negative regulation of peptidyl-threonine phosphorylation [TAS]
- negative regulation of ryanodine-sensitive calcium-release channel activity [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- nitric oxide metabolic process [TAS]
- phototransduction, visible light [TAS]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of cyclic nucleotide metabolic process [IDA]
- positive regulation of cyclic-nucleotide phosphodiesterase activity [IDA]
- positive regulation of peptidyl-threonine phosphorylation [TAS]
- positive regulation of phosphoprotein phosphatase activity [IDA]
- positive regulation of protein autophosphorylation [TAS]
- positive regulation of protein dephosphorylation [IDA]
- positive regulation of protein serine/threonine kinase activity [TAS]
- positive regulation of ryanodine-sensitive calcium-release channel activity [IDA]
- regulation of cardiac muscle contraction [IMP]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [IC]
- regulation of cell communication by electrical coupling involved in cardiac conduction [IC]
- regulation of cytokinesis [IMP]
- regulation of heart rate [IMP]
- regulation of nitric-oxide synthase activity [TAS]
- regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum [IDA]
- regulation of rhodopsin mediated signaling pathway [TAS]
- response to calcium ion [IDA]
- rhodopsin mediated signaling pathway [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- substantia nigra development [IEP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function- N-terminal myristoylation domain binding [IPI]
- calcium ion binding [IDA, ISS]
- ion channel binding [IPI]
- phospholipase binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase activator activity [IDA]
- protein serine/threonine kinase activator activity [TAS]
- thioesterase binding [IPI]
- titin binding [IPI]
- N-terminal myristoylation domain binding [IPI]
- calcium ion binding [IDA, ISS]
- ion channel binding [IPI]
- phospholipase binding [IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase activator activity [IDA]
- protein serine/threonine kinase activator activity [TAS]
- thioesterase binding [IPI]
- titin binding [IPI]
Gene Ontology Cellular Component
Homo sapiens
PREY
STX4
STX4A, p35-2
syntaxin 4
GO Process (23)
GO Function (4)
GO Component (18)
Gene Ontology Biological Process
- blood coagulation [TAS]
- intracellular protein transport [IBA]
- long-term synaptic potentiation [IDA]
- membrane organization [TAS]
- organelle fusion [IDA]
- platelet activation [TAS]
- positive regulation of catalytic activity [IMP]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell migration [IMP]
- positive regulation of cell proliferation [IMP]
- positive regulation of chemotaxis [IMP]
- positive regulation of eosinophil degranulation [IMP]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of immunoglobulin secretion [IMP]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [IDA, IMP]
- positive regulation of protein localization to cell surface [IMP]
- positive regulation of protein localization to plasma membrane [IMP]
- post-Golgi vesicle-mediated transport [TAS]
- regulation of exocytosis [IMP]
- regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- response to hydroperoxide [IDA]
- synaptic vesicle fusion to presynaptic membrane [IBA]
- vesicle docking [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- SNARE complex [IDA]
- basolateral plasma membrane [IDA]
- cell surface [IDA]
- cytosol [TAS]
- dendritic spine [IDA]
- endosome [IDA]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- integral component of membrane [IBA]
- intracellular [IDA]
- lamellipodium [IDA]
- membrane [IDA]
- plasma membrane [IDA, TAS]
- somatodendritic compartment [IDA]
- specific granule [IDA]
- synapse [IDA]
- synaptic vesicle [IBA]
- vacuole [TAS]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID