Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The bHLH genes GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root.

Bernhardt C, Lee MM, Gonzalez A, Zhang F, Lloyd A, Schiefelbein J

The position-dependent specification of the hair and non-hair cell types in the Arabidopsis root epidermis provides a simple model for the study of cell fate determination in plants. Several putative transcriptional regulators are known to influence this cell fate decision. Indirect evidence from studies with the maize R gene has been used to suggest that a bHLH transcription factor also ... [more]

Development Dec. 01, 2003; 130(26);6431-9 [Pubmed: 14627722]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CPC EGL3	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Song SK (2011)	Low	-	BioGRID	-
EGL3 CPC	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Zhang F (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EGL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

CPC