BAIT
FARP1
CDEP, FARP1-IT1, PLEKHC2, PPP1R75, RP11-111L24.1
FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
GO Process (5)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
SDC3
SDCN, SYND3
syndecan 3
GO Process (9)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- chondroitin sulfate metabolic process [TAS]
- extracellular matrix organization [TAS]
- glycosaminoglycan biosynthetic process [TAS]
- glycosaminoglycan catabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- phototransduction, visible light [TAS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID