BAIT
G3BP2
GTPase activating protein (SH3 domain) binding protein 2
GO Process (2)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
MTPN
GCDP, V-1
myotrophin
GO Process (10)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- cell growth [IDA]
- neuron differentiation [NAS]
- positive regulation of NF-kappaB transcription factor activity [ISS]
- positive regulation of cardiac muscle hypertrophy [IMP]
- positive regulation of cell growth [ISS]
- positive regulation of macromolecule biosynthetic process [ISS]
- positive regulation of protein metabolic process [ISS]
- regulation of barbed-end actin filament capping [IDA]
- regulation of striated muscle tissue development [NAS]
- regulation of translation [NAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID