BAIT

GDI1

1A, GDIL, MRX41, MRX48, OPHN2, RABGD1A, RABGDIA, XAP-4, XX-FW81657B9.1

GDP dissociation inhibitor 1

GO Process (6)

GO Function (3)

GO Component (3)

Gene Ontology Biological Process

Rab protein signal transduction [ISS]

negative regulation of axonogenesis [ISS]

negative regulation of protein targeting to membrane [IMP]

regulation of small GTPase mediated signal transduction [TAS]

signal transduction [TAS]

small GTPase mediated signal transduction [TAS]

Gene Ontology Molecular Function

GDP-dissociation inhibitor activity [TAS]
Rab GDP-dissociation inhibitor activity [ISS]
protein binding [IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

TAP1

ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4, TAP1*0102N, TAP1N, DAAP-57C1.5

transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)

GO Process (8)

GO Function (10)

GO Component (5)

Gene Ontology Biological Process

antigen processing and presentation of endogenous peptide antigen via MHC class I [IMP]

antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]

antigen processing and presentation of peptide antigen via MHC class I [TAS]

cytosol to ER transport [IMP]

intracellular transport of viral protein in host cell [IMP]

peptide transport [IMP]

transmembrane transport [IBA]

Gene Ontology Molecular Function

ADP binding [IDA]
ATP binding [IDA]
ATPase activity, coupled to transmembrane movement of substances [IBA]
MHC class Ib protein binding [IPI]
TAP1 binding [ISS]
TAP2 binding [IPI]
peptide antigen binding [NAS]
peptide transporter activity [IGI, IMP]
protein binding [IPI]
protein homodimerization activity [ISS]

Gene Ontology Cellular Component

TAP complex [IDA]

endoplasmic reticulum membrane [TAS]

integral component of endoplasmic reticulum membrane [IDA]

integral component of membrane [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

OpenCell: Endogenous tagging for the cartography of human cellular organization.

Cho NH, Cheveralls KC, Brunner AD, Kim K, Michaelis AC, Raghavan P, Kobayashi H, Savy L, Li JY, Canaj H, Kim JYS, Stewart EM, Gnann C, McCarthy F, Cabrera JP, Brunetti RM, Chhun BB, Dingle G, Hein MY, Huang B, Mehta SB, Weissman JS, Gomez-Sjoeberg R, Itzhak DN, Royer LA, Mann M, Leonetti MD

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]

Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]

Throughput

High Throughput

Additional Notes

Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins

Curated By

BioGRID