CDC6
Gene Ontology Biological Process
- DNA replication [TAS]
- DNA replication checkpoint [TAS]
- G1/S transition of mitotic cell cycle [TAS]
- mitotic cell cycle [TAS]
- negative regulation of DNA replication [TAS]
- negative regulation of cell proliferation [TAS]
- positive regulation of chromosome segregation [IDA]
- positive regulation of cytokinesis [IMP]
- regulation of cyclin-dependent protein serine/threonine kinase activity [TAS]
- regulation of mitotic metaphase/anaphase transition [IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [TAS]
- traversing start control point of mitotic cell cycle [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MCM8
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Involvement of human MCM8 in prereplication complex assembly by recruiting hcdc6 to chromatin.
The MCM2-MCM7 complex is an essential component of the prereplication complex (pre-RC), which is recruited by the cdc6 and cdt1 proteins to origins of DNA replication during G(1) phase. Here, we report that the accumulation on chromatin of another member of the MCM protein family, human MCM8 (hMCM8), occurs during early G(1) phase, before the hMCM2-hMCM7 complex binds. hMCM8 interacts ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MCM8 CDC6 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID