BAIT
HSPH1
HSP105, HSP105A, HSP105B, NY-CO-25, RP11-173P16.1
heat shock 105kDa/110kDa protein 1
GO Process (3)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
STXBP1
MUNC18-1, NSEC1, P67, RBSEC1, UNC18, RP11-56D16.3
syntaxin binding protein 1
GO Process (15)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- axon target recognition [ISS]
- energy reserve metabolic process [TAS]
- glutamate secretion [TAS]
- negative regulation of synaptic transmission, GABAergic [ISS]
- neurotransmitter secretion [TAS]
- platelet aggregation [IMP]
- platelet degranulation [IMP]
- protein localization to plasma membrane [IDA]
- regulation of SNARE complex assembly [TAS]
- regulation of insulin secretion [TAS]
- regulation of synaptic vesicle fusion to presynaptic membrane [TAS]
- regulation of synaptic vesicle priming [ISS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
- synaptic vesicle maturation [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID