INTS5
Gene Ontology Biological Process
Gene Ontology Molecular Function
POLR2K
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- DNA repair [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- innate immune response [TAS]
- mRNA splicing, via spliceosome [TAS]
- nucleotide-excision repair [TAS]
- positive regulation of type I interferon production [TAS]
- positive regulation of viral transcription [TAS]
- regulation of transcription from RNA polymerase I promoter [TAS]
- termination of RNA polymerase I transcription [TAS]
- termination of RNA polymerase III transcription [TAS]
- transcription elongation from RNA polymerase I promoter [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription elongation from RNA polymerase III promoter [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription from RNA polymerase II promoter [IDA, TAS]
- transcription from RNA polymerase III promoter [TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| INTS5 POLR2K | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -3.7456 | BioGRID | 2457561 |
Curated By
- BioGRID