BAIT
LMNB1
ADLD, LMN, LMN2, LMNB
lamin B1
GO Process (2)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Homo sapiens
PREY
NIPBL
CDLS, CDLS1, IDN3, IDN3-B, Scc2
Nipped-B homolog (Drosophila)
GO Process (28)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
- brain development [IMP]
- cellular protein localization [IMP]
- cellular response to DNA damage stimulus [IMP]
- cellular response to X-ray [IMP]
- cognition [IMP]
- developmental growth [IMP]
- ear morphogenesis [IMP]
- embryonic digestive tract morphogenesis [IMP]
- embryonic forelimb morphogenesis [IMP]
- external genitalia morphogenesis [IMP]
- eye morphogenesis [IMP]
- face morphogenesis [IMP]
- forelimb morphogenesis [IMP]
- gall bladder development [IMP]
- heart morphogenesis [IMP]
- maintenance of mitotic sister chromatid cohesion [IMP]
- metanephros development [NAS]
- mitotic cell cycle [TAS]
- mitotic sister chromatid cohesion [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- outflow tract morphogenesis [IMP]
- positive regulation of histone deacetylation [IDA]
- regulation of developmental growth [IMP]
- regulation of embryonic development [IMP]
- regulation of hair cycle [IMP]
- sensory perception of sound [IMP]
- uterus morphogenesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID