An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

OpenCell: Endogenous tagging for the cartography of human cellular organization.

Cho NH, Cheveralls KC, Brunner AD, Kim K, Michaelis AC, Raghavan P, Kobayashi H, Savy L, Li JY, Canaj H, Kim JYS, Stewart EM, Gnann C, McCarthy F, Cabrera JP, Brunetti RM, Chhun BB, Dingle G, Hein MY, Huang B, Mehta SB, Weissman JS, Gomez-Sjoeberg R, Itzhak DN, Royer LA, Mann M, Leonetti MD

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]

Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]

Throughput

High Throughput

Additional Notes

Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STRN MAP4K4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1449409
STRN MAP4K4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9689	BioGRID	3160767
MAP4K4 STRN	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Seo G (2020)	Low	-	BioGRID	-
STRN MAP4K4	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Seo G (2020)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

MAP4K4

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]receptor signaling protein serine/threonine kinase activity [IBA]

Gene Ontology Cellular Component

STRN

Gene Ontology Biological Process

Gene Ontology Molecular Function

armadillo repeat domain binding [IPI]calmodulin binding [ISS, NAS]estrogen receptor binding [IPI]protein binding [IPI]protein complex binding [IDA]protein phosphatase 2A binding [IDA]