An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

OpenCell: Endogenous tagging for the cartography of human cellular organization.

Cho NH, Cheveralls KC, Brunner AD, Kim K, Michaelis AC, Raghavan P, Kobayashi H, Savy L, Li JY, Canaj H, Kim JYS, Stewart EM, Gnann C, McCarthy F, Cabrera JP, Brunetti RM, Chhun BB, Dingle G, Hein MY, Huang B, Mehta SB, Weissman JS, Gomez-Sjoeberg R, Itzhak DN, Royer LA, Mann M, Leonetti MD

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]

Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]

Throughput

High Throughput

Additional Notes

Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MAPRE1 APC	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bouguenina H (2017)	High	-	BioGRID	-
MAPRE1 APC	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1443138
MAPRE1 APC	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zhang Y (2016)	Low	-	BioGRID	-
MAPRE1 APC	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Go CD (2021)	High	16.75	BioGRID	2995709
APC MAPRE1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Askham JM (2000)	Low	-	BioGRID	-
APC MAPRE1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Askham JM (2002)	Low	-	BioGRID	-
MAPRE1 APC	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Xia P (2012)	Low	-	BioGRID	1054145

Curated By

BioGRID

Interaction Summary

MAPRE1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule plus-end binding [IDA]poly(A) RNA binding [IDA]protein C-terminus binding [TAS]protein binding [IPI]

Gene Ontology Cellular Component

APC

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-catenin binding [IPI]cadherin binding [IDA]gamma-catenin binding [IPI]microtubule binding [IDA]microtubule plus-end binding [IDA]protein binding [IPI]protein kinase binding [IPI]protein kinase regulator activity [IDA]