BAIT
MTMR2
CMT4B, CMT4B1
myotubularin related protein 2
GO Process (11)
GO Function (4)
GO Component (10)
Gene Ontology Biological Process
- dendritic spine maintenance [ISS]
- negative regulation of endocytosis [ISS]
- negative regulation of excitatory postsynaptic membrane potential [ISS]
- negative regulation of receptor catabolic process [ISS]
- negative regulation of receptor internalization [ISS]
- phosphatidylinositol biosynthetic process [TAS]
- phosphatidylinositol dephosphorylation [IDA]
- phospholipid metabolic process [TAS]
- positive regulation of early endosome to late endosome transport [ISS]
- protein dephosphorylation [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
G6PD
G6PD1
glucose-6-phosphate dehydrogenase
GO Process (18)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- NADP metabolic process [IDA]
- NADPH regeneration [IMP]
- carbohydrate metabolic process [TAS]
- cellular response to oxidative stress [IMP]
- cholesterol biosynthetic process [IMP]
- cytokine production [IMP]
- erythrocyte maturation [IMP]
- glucose 6-phosphate metabolic process [IDA, IMP]
- glutathione metabolic process [IMP]
- lipid metabolic process [TAS]
- negative regulation of protein glutathionylation [IMP]
- oxidation-reduction process [IMP]
- pentose biosynthetic process [IDA]
- pentose-phosphate shunt [IDA, TAS]
- pentose-phosphate shunt, oxidative branch [IMP]
- ribose phosphate biosynthetic process [IMP]
- small molecule metabolic process [TAS]
- substantia nigra development [IEP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID