BAIT
MYO9B
CELIAC4, MYR5
myosin IXB
GO Process (5)
GO Function (9)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DICER1
DCR1, Dicer, Dicer1e, HERNA, K12H4.8-LIKE, MNG1, RMSE2
dicer 1, ribonuclease type III
GO Process (15)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- RNA phosphodiester bond hydrolysis [IDA]
- RNA phosphodiester bond hydrolysis, endonucleolytic [IDA, IMP]
- conversion of ds siRNA to ss siRNA [IMP]
- gene expression [TAS]
- negative regulation of Schwann cell proliferation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- nerve development [ISS]
- neuron projection morphogenesis [ISS]
- peripheral nervous system myelin formation [ISS]
- positive regulation of Schwann cell differentiation [ISS]
- positive regulation of myelination [ISS]
- pre-miRNA processing [IDA]
- production of miRNAs involved in gene silencing by miRNA [ISS]
- production of siRNA involved in RNA interference [IDA]
- targeting of mRNA for destruction involved in RNA interference [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID