BAIT

ZMYM2

FIM, MYM, RAMP, SCLL, ZNF198, RP11-264J4.7
zinc finger, MYM-type 2
GO Process (0)
GO Function (2)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens
PREY

FUS

ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS
FUS RNA binding protein
GO Process (3)
GO Function (4)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mass spectroscopy identifies the splicing-associated proteins, PSF, hnRNP H3, hnRNP A2/B1, and TLS/FUS as interacting partners of the ZNF198 protein associated with rearrangement in myeloproliferative disease.

Kasyapa CS, Kunapuli P, Cowell JK

ZNF198 is fused with FGFR1 in an atypical myeloproliferative disease that results in constitutive activation of the kinase domain and mislocalization to the cytoplasm. We have used immunoprecipitation of a GFP-tagged ZNF198 combined with MALDI-TOF mass spectroscopy to identify interacting proteins. P splicing factor (PSF) was identified as one of the proteins and this interaction was confirmed by Western blotting. ... [more]

Exp. Cell Res. Sep. 10, 2005; 309(1);78-85 [Pubmed: 15975576]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ZMYM2 FUS
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.7215BioGRID
3288295

Curated By

  • BioGRID