BAIT
PI4K2A
PI4KII, PIK42A, RP11-548K23.6
phosphatidylinositol 4-kinase type 2 alpha
GO Process (4)
GO Function (5)
GO Component (16)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- BLOC-1 complex [IDA]
- cytoplasmic vesicle [ISS]
- cytosol [TAS]
- dendrite [ISS]
- endosome [ISS]
- growing cell tip [ISS]
- host cell presynaptic membrane [ISS]
- integral component of plasma membrane [IDA]
- intrinsic component of membrane [IDA]
- lysosomal membrane [IDA]
- membrane [IDA]
- membrane raft [IDA]
- mitochondrion [ISS]
- neuron projection [ISS]
- neuronal cell body [ISS]
- presynaptic membrane [ISS]
Homo sapiens
PREY
ALDH1A1
ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, RP11-151D14.2
aldehyde dehydrogenase 1 family, member A1
GO Process (5)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID