BAIT
PIK3C2A
CPK, PI3-K-C2(ALPHA), PI3-K-C2A
phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha
GO Process (10)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
- clathrin coat assembly [TAS]
- endocytosis [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- exocytosis [TAS]
- insulin receptor signaling pathway [TAS]
- phosphatidylinositol biosynthetic process [TAS]
- phospholipid metabolic process [TAS]
- platelet-derived growth factor receptor signaling pathway [TAS]
- small molecule metabolic process [TAS]
- vascular smooth muscle contraction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PFN1
ALS18
profilin 1
GO Process (10)
GO Function (6)
GO Component (5)
Gene Ontology Biological Process
- blood coagulation [TAS]
- negative regulation of actin filament bundle assembly [IMP]
- negative regulation of actin filament polymerization [IDA]
- negative regulation of stress fiber assembly [IMP]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of ATPase activity [IDA]
- positive regulation of actin filament polymerization [IGI]
- positive regulation of epithelial cell migration [IMP]
- positive regulation of ruffle assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID