BAIT
POLR1C
RPA39, RPA40, RPA5, RPAC1, TCS3, RP3-337H4.4
polymerase (RNA) I polypeptide C, 30kDa
GO Process (10)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- gene expression [TAS]
- innate immune response [TAS]
- positive regulation of type I interferon production [TAS]
- termination of RNA polymerase I transcription [TAS]
- termination of RNA polymerase III transcription [TAS]
- transcription elongation from RNA polymerase I promoter [TAS]
- transcription elongation from RNA polymerase III promoter [TAS]
- transcription from RNA polymerase I promoter [TAS]
- transcription from RNA polymerase III promoter [TAS]
- transcription initiation from RNA polymerase I promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SNX3
Grd19, MCOPS8, SDP3, RP3-429G5.4
sorting nexin 3
GO Process (12)
GO Function (6)
GO Component (11)
Gene Ontology Biological Process
- intralumenal vesicle formation [IMP]
- membrane invagination [IDA]
- negative regulation of early endosome to late endosome transport [IDA]
- negative regulation of phagocytosis [IMP]
- negative regulation of protein catabolic process [IDA]
- negative regulation of protein transport [IDA]
- negative regulation of viral entry into host cell [IDA]
- positive regulation of neuron projection development [ISS]
- protein to membrane docking [IDA]
- regulation of Wnt signaling pathway [IMP]
- response to bacterium [IDA]
- vesicle organization [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID