BAIT

NIMIN1

NIM1-interacting 1, NIMIN-1, T6A9.23, AT1G02450
protein NIM1-INTERACTING 1
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

NPR1

ARABIDOPSIS NONEXPRESSER OF PR GENES 1, ATNPR1, F15H21.6, F15H21_6, NIM1, NON-INDUCIBLE IMMUNITY 1, NONEXPRESSER OF PR GENES 1, REGULATORY PROTEIN NPR1, SAI1, SALICYLIC ACID INSENSITIVE 1, AT1G64280
Regulatory protein NPR1
Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of NIMIN1 with NPR1 modulates PR gene expression in Arabidopsis.

Weigel RR, Pfitzner UM, Gatz C

The Arabidopsis thaliana NONEXPRESSER OF PR GENES1 (NPR1, also known as NIM1) protein is an essential positive regulator of salicylic acid (SA)-induced PATHOGENESIS-RELATED (PR) gene expression and systemic acquired resistance (SAR). PR gene activity is regulated at the level of redox-dependent nuclear transport of NPR1. NPR1 interacts with members of the TGA family of transcription factors that are known to ... [more]

Plant Cell Apr. 01, 2005; 17(4);1279-91 [Pubmed: 15749762]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NIMIN1 NPR1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
NIMIN1 NPR1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
NIMIN1 NPR1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID