BAIT

ROP1

ARABIDOPSIS THALIANA RHO-RELATED PROTEIN FROM PLANTS 1, ARAC11, ATRAC11, ATROP1, Arabidopsis RAC-like 11, RHO-related protein from plants 1, ROP1AT, AT3G51300
Rac-like GTP-binding protein ARAC11
Arabidopsis thaliana (Columbia)
PREY

RIC4

MQK4.23, MQK4_23, ROP-interactive CRIB motif-containing protein 4, AT5G16490
ROP-interactive CRIB motif-containing protein 4
GO Process (5)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

A Rho family GTPase controls actin dynamics and tip growth via two counteracting downstream pathways in pollen tubes.

Gu Y, Fu Y, Dowd P, Li S, Vernoud V, Gilroy S, Yang Z

Tip growth in neuronal cells, plant cells, and fungal hyphae is known to require tip-localized Rho GTPase, calcium, and filamentous actin (F-actin), but how they interact with each other is unclear. The pollen tube is an exciting model to study spatiotemporal regulation of tip growth and F-actin dynamics. An Arabidopsis thaliana Rho family GTPase, ROP1, controls pollen tube growth by ... [more]

J. Cell Biol. Apr. 11, 2005; 169(1);127-38 [Pubmed: 15824136]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ROP1 RIC4
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
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Curated By

  • BioGRID