BAIT

DL1

ADL1, ADL1A, AG68, DRP1A, DYNAMIN-RELATED PROTEIN 1A, MJC20.19, MJC20_19, RADIAL SWELLING 9, RSW9, dynamin-like protein, AT5G42080
dynamin-related protein 1A
Arabidopsis thaliana (Columbia)
PREY

SFC

AGD3, ARF-GAP DOMAIN3, ASCULAR NETWORK DEFECTIVE 3, SCARFACE, T31B5.120, T31B5_120, VAN3, AT5G13300
ADP-ribosylation factor GTPase-activating protein AGD3
Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

DRP1A is responsible for vascular continuity synergistically working with VAN3 in Arabidopsis.

Sawa S, Koizumi K, Naramoto S, Demura T, Ueda T, Nakano A, Fukuda H

In most dicotyledonous plants, vascular tissues in the leaf have a reticulate venation pattern. We have isolated and characterized an Arabidopsis (Arabidopsis thaliana) mutant defective in the vascular network defective mutant, van3. van3 mutants show a discontinuous vascular pattern, and VAN3 is known to encode an ADP-ribosylation-factor-GTPase-activating protein that regulates membrane trafficking in the trans-Golgi network. To elucidate the molecular ... [more]

Plant Physiol. Jun. 01, 2005; 138(2);819-26 [Pubmed: 15923323]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SFC DL1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
DL1 SFC
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID