Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Characterization of proteins that interact with the GTP-bound form of the regulatory GTPase Ran in Arabidopsis.

Haizel T, Merkle T, Pay A, Fejes E, Nagy F

Ran, a small soluble GTP-binding protein, has been shown to be essential for the nuclear translocation of proteins and it is also thought to be involved in regulating cell cycle progression in mammalian and yeast cells. Genes encoding Ran-like proteins have been isolated from different higher plant species. Overexpression of plant Ran cDNAs, similarly to their mammalian/yeast homologues, suppresses the ... [more]

Plant J. Jan. 01, 1997; 11(1);93-103 [Pubmed: 9025305]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SIRANBP RAN3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Braun P (2011)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

RAN3

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [ISS]GTPase activity [ISS]protein binding [IPI]

Gene Ontology Cellular Component

SIRANBP