SACM1L
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAPA
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- cell death [IMP]
- endoplasmic reticulum organization [IMP]
- membrane fusion [TAS]
- negative regulation by host of viral genome replication [IDA]
- neuron projection development [IMP]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- protein folding in endoplasmic reticulum [IMP]
- protein localization to endoplasmic reticulum [IMP]
- signal transduction [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VAPA SACM1L | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3374752 | |
| SACM1L VAPA | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - |
Curated By
- BioGRID