SAR1B
Gene Ontology Biological Process
- COPII vesicle coating [TAS]
- ER to Golgi vesicle-mediated transport [TAS]
- GTP catabolic process [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cellular protein metabolic process [TAS]
- membrane organization [TAS]
- post-translational protein modification [TAS]
- protein N-linked glycosylation via asparagine [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GAPDH
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular response to interferon-gamma [IDA]
- gluconeogenesis [TAS]
- glucose metabolic process [TAS]
- glycolytic process [TAS]
- microtubule cytoskeleton organization [ISS]
- negative regulation of translation [IDA, IMP]
- neuron apoptotic process [ISS]
- peptidyl-cysteine S-trans-nitrosylation [ISS]
- protein stabilization [ISS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [IDA]
- cytoplasm [IDA, ISS]
- cytosol [IDA, ISS, TAS]
- extracellular vesicular exosome [IDA]
- intracellular membrane-bounded organelle [IDA]
- lipid particle [IDA]
- membrane [IDA]
- microtubule cytoskeleton [ISS]
- nuclear membrane [IDA]
- nucleus [IDA, ISS]
- plasma membrane [IDA]
- ribonucleoprotein complex [IDA]
- vesicle [IDA]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
SAR1B GAPDH | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3412313 |
Curated By
- BioGRID