A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana.

Desprez T, Juraniec M, Crowell EF, Jouy H, Pochylova Z, Parcy F, Hoefte H, Gonneau M, Vernhettes S

In all land plants, cellulose is synthesized from hexameric plasma membrane complexes. Indirect evidence suggests that in vascular plants the complexes involved in primary wall synthesis contain three distinct cellulose synthase catalytic subunits (CESAs). In this study, we show that CESA3 and CESA6 fused to GFP are expressed in the same cells and at the same time in the hypocotyl ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 25, 2007; 104(39);15572-7 [Pubmed: 17878303]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CESA1 CESA6	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gonneau M (2014)	Low	-	BioGRID	-
CESA6 CESA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gonneau M (2014)	Low	-	BioGRID	-
CESA6 CESA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lampugnani ER (2024)	High	-	BioGRID	3654500
CESA6 CESA1	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Desprez T (2007)	Low	-	BioGRID	-
CESA1 CESA6	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Carroll A (2012)	Low	-	BioGRID	-
CESA6 CESA1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Carroll A (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CESA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cellulose synthase activity [ISS]transferase activity, transferring glycosyl groups [ISS]

Gene Ontology Cellular Component

CESA6