A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Arabidopsis MEKK1 can take a short cut: it can directly interact with senescence-related WRKY53 transcription factor on the protein level and can bind to its promoter.

Miao Y, Laun TM, Smykowski A, Zentgraf U

Despite the importance of the senescence processes in plants, our knowledge on regulatory mechanisms of senescence is still poor. WRKY transcription factors have been shown to be involved in the regulation of leaf senescence. However, almost nothing is known about the upstream regulation of the senescence specific expression of WRKY factors. Therefore, we characterized proteins that bind and activate the ... [more]

Plant Mol. Biol. Sep. 01, 2007; 65(1);63-76 [Pubmed: 17587183]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MEKK1 WRKY53	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Miao Y (2007)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

WRKY53

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]protein binding [IPI]sequence-specific DNA binding transcription factor activity [IMP, ISS]

Gene Ontology Cellular Component

MEKK1