BAIT

HXK1

ARABIDOPSIS THALIANA HEXOKINASE 1, ATHXK1, F19B15.160, F19B15_160, GIN2, GLUCOSE INSENSITIVE 2, HEXOKINASE, hexokinase 1, AT4G29130
hexokinase 1
Arabidopsis thaliana (Columbia)
PREY

RPT5B

26S proteasome AAA-ATPase subunit RPT5B, F7G19.2, F7G19_2, AT1G09100
26S proteasome AAA-ATPase subunit RPT5B
GO Process (4)
GO Function (2)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Regulatory functions of nuclear hexokinase1 complex in glucose signaling.

Cho YH, Yoo SD, Sheen J

Arabidopsis hexokinase1 (HXK1) is a glucose sensor that integrates nutrient and hormone signals to govern gene expression and plant growth in response to environmental cues. How the metabolic enzyme mediates glucose signaling remains a mystery. By coupling proteomic and binary-interaction screens, we discover two nuclear-specific HXK1 unconventional partners: the vacuolar H(+)-ATPase B1 (VHA-B1) and the 19S regulatory particle of proteasome ... [more]

Cell Nov. 03, 2006; 127(3);579-89 [Pubmed: 17081979]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HXK1 RPT5B
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
HXK1 RPT5B
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID