SUPT5H
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- DNA-templated transcription, elongation [IDA]
- cell cycle [NAS]
- chromatin remodeling [NAS]
- gene expression [TAS]
- negative regulation of DNA-templated transcription, elongation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA-templated transcription, elongation [IDA]
- positive regulation of macroautophagy [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of viral transcription [TAS]
- response to organic substance [TAS]
- single stranded viral RNA replication via double stranded DNA intermediate [NAS]
- transcription elongation from RNA polymerase II promoter [IDA, TAS]
- transcription from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DSIF complex [IDA]
- nucleoplasm [IDA, TAS]
- nucleus [IDA]
POLR2B
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [TAS]
- DNA repair [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA splicing, via spliceosome [TAS]
- nucleotide-excision repair [TAS]
- positive regulation of viral transcription [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [IDA, TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription-coupled nucleotide-excision repair [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| POLR2B SUPT5H | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3363207 | |
| SUPT5H POLR2B | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1446206 | |
| SUPT5H POLR2B | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.461 | BioGRID | 1266944 | |
| SUPT5H POLR2B | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | Low | - | BioGRID | 2816560 |
Curated By
- BioGRID