BAIT
SYAP1
PRO3113
synapse associated protein 1
GO Process (0)
GO Function (0)
GO Component (4)
Gene Ontology Cellular Component
Homo sapiens
PREY
MED25
ACID1, ARC92, CMT2B2, P78, PTOV2, TCBAP0758
mediator complex subunit 25
GO Process (8)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
- gene expression [TAS]
- negative regulation of fibroblast proliferation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of cell cycle arrest [IMP]
- positive regulation of chromatin binding [IMP]
- positive regulation of mediator complex assembly [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Science Dec. 11, 2021; 375(6585);eabi6983 [Pubmed: 35271311]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Curated By
- BioGRID